Fig. 7.

Src and TACE are activated in overloaded mouse soleus. The gastrocnemius of adult male mice (C57BL/6) was severed bilaterally to create functional overloading of soleus and plantaris. Sham-operated mice were used as control (day 0). At the indicated number of days after the surgery, the soleus was collected for western blot analysis of activation of Src (A), TACE (B) and p38 (C), as well as expression of myogenin (D). Each sample shown represents one mouse. Bar graphs summarize optical density data of the activated forms of the proteins normalized against the total protein or GAPDH. Data were analyzed by ANOVA. *P<0.05 compared with control (day 0). For D, the bar graph used arbitrary units owing to the zero expression of myogenin on day 0. (E) Cross sections of soleus prepared from mice that had been sham-operated or overloaded for 5 days were examined by immunohistofluorescence staining to visualize the localization of active Src (Red). Scale bars: 20 µm.