Fig. 3.

CNG channel subunits are localized to distinct ciliary subdomains in a cell-specific manner. Colocalization of TAX-2::GFP, TAX-4::GFP and CNG-3::mCherry protein fusions in AWB (A) and TAX-2::GFP, TAX-4::GFP and CNG-3::GFP in ASK (B) cilia of adult hermaphrodites with the indicated fusion proteins marking ciliary subcompartments. Anterior is left. Scale bar: 5 µm. (C) Cartoons summarize localization patterns of examined CNG proteins in AWB and ASK cilia. Depicted channel subunit stoichiometry is hypothetical but based on previous observations showing that TAX-4, but not TAX-2, can form functional homomeric channels, and that TAX-2 and TAX-4 can heterotetramerize (Komatsu et al., 1999; Komatsu et al., 1996). TZ, transition zone; BB, basal body (Reiter et al., 2012). The middle and distal segments are defined as ciliary domains that contain doublet or singlet microtubules, respectively; the far-distal segment in AWB cilia was defined as a region that did not appear to contain an axoneme (Mukhopadhyay et al., 2007; Perkins et al., 1986; Ward et al., 1975).