Fig. 6.

Sequences in TAX-2 and TAX-4 required for ciliary localization. (A,B) Expression of GFP-tagged full-length or truncated TAX-2 (A) or TAX-4 (B) proteins in AWB and ASK. Proteins were expressed in AWB and ASK under the str-1 or srbc-66 promoters, respectively. S1–S6, transmembrane regions; P, P loop; CNBD, cNMP binding domain; LZ, leucine zipper; no exp., no expression; MS, middle segments. Lack of expression of mutated proteins could result from altered protein trafficking, stability or membrane targeting. (C,D) Expression of indicated chimeric fusion proteins in AWB. (E) Localization pattern of TAX-4/2::GFP in AWB cilia. The TAX-4/2::GFP fusion protein and mCherry were driven under the str-1 promoter. Quantification of average relative fluorescence intensities of full-length TAX-2::GFP, full-length TAX-4:GFP and TAX-4/2::GFP across AWB cilia are shown on the right. Arrow indicates point of initiation of fluorescence intensity measurements. Distribution of TAX-4/2 is different from that of TAX-4 at P<0.001, but statistically indistinguishable from that of TAX-2 (ANOVA and post-hoc correction for multiple comparisons). n = 10 cilia each. Scale bar: 5 µm.