Table 2. Targeting and localization of TAX-2 and TAX-4 to AWB and ASK cilia require distinct trans-acting factors.

^aTAX-2 and TAX-4 were fused to GFP. Expression in AWB and ASK was driven under the str-1 and srbc-66 promoters, respectively. Strains also carry CHE-13::tagRFP or mCherry fluorescent proteins expressed under the relevant cell-specific promoter.

^bMS, middle segment.

^cDifferences among proportions in different categories were compared to respective wild-type values in the relevant neuron type for statistical significance unless indicated otherwise. P values were determined using a χ² test of independence. Only differences at P<0.05 or lower are indicated.

^dCompared with values in nphp-2 mutants.

^eCompared with values in kap-1 mutants.

Adult animals grown at 20°C were examined. n = 20–60 animals each. Expression from the same array was examined in wild-type and mutant animals with the exception of the mks-1; mksr-2; mksr-1 triple mutant strain which was injected with the CNG channel subunit fusion constructs. In all cases, with the exception of osm-3 mutants, localization was visualized only in animals exhibiting normal overall ciliary morphology.