Fig. 1.

Proposed CaM-binding sites in the primary sequences of RyR1 and RyR2. Regions proposed for Ca²⁺-CaM binding are shown as red boxes, whereas regions proposed for both apo-CaM and Ca²⁺-CaM binding are shown as pink boxes. [Figure adapted from Balshaw et al. (Balshaw et al., 2001) with modification.] The two green circles indicate positions where GFP was inserted (see Materials and Methods) into the sequence of RyR2 in two of the proposed CaM-binding regions, specifically, after residues Arg3595 and Lys4269.