Fig. 2.

Analysis of LAMP products. a Naked-eye-visualization of HNB dye colored RT-LAMP amplified products of Human RVA clinical samples. Tubes1, 2, 4, 5 Human RVA positive samples (sky blue color); Tube3, 6, 7 Human RVA negative samples (violet color); Tube8 no template control (NTC). b Restriction enzyme analysis of LAMP products in 1.5 % agarose gel. Lane M 100-bp DNA ladder (Fermentas); Lane 1 LAMP products of Human RVA NSP4 gene without AluI digestion; Lane 2 LAMP products digested with AluI (arrowpoints 89 and 117 bp digests). (Color figure online)