Fig. 4.

Sensitivity comparison of conventional RT-PCR and RT-LAMP assays using human RVA partial length NSP4 gene plasmid construct. aLaneM 100-bp DNA ladder (Fermentas); Lanes2–8 LAMP products using plasmid log dilutions starting from 1 log to 7 log dilutions. bLaneM 100-bp DNA ladder (Fermentas); Lanes 2–8 RT-PCR products using plasmid log dilutions starting from 1 log to 7 log dilutions. The RT-PCR amplicon size (206 bp) is in good agreement with the expected product size. All products were electrophoresed on a 1.5 % agarose gel