Table 2.
Genetic transformation and regeneration of peanut explants from the cultivars K6 and K134
| No. of co-cultivations | Total explants co-cultured | Explants regenerated | Shoots produced | Shoots passed antibiotic selection | Shoots produced roots | Plantlets hardened | Plants survived in glasshouse | Final recovery of putative transgenics |
|---|---|---|---|---|---|---|---|---|
| cv. K6 | ||||||||
| 10 (DC) | 409 | 247 | 3,938 | 1,229 | 1,038 | 1,010 | 919 | 84 (34.0) |
| 6 (IL) | 1,028 | 796 | 2,263 | 746 | 538 | 368 | 301 | 9 (0.9) |
| cv. K134 | ||||||||
| 5 (DC) | 108 | 91 | 932 | 484 | 280 | 238 | 205 | 15 (16.5) |
| 3 (IL) | 459 | 353 | 273 | 82 | 65 | 51 | 41 | 8 (2.2) |
Values in parenthesis are percentage over explants regenerated
DC De-embryonated cotyledons, IL immature leaves