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PCR and Southern analysis of the peanut transgenic lines to confirm the integration of the TSV-CP transgene
| Variety | Total | ||||
|---|---|---|---|---|---|
| K6 | K134 | ||||
| DC | IL | DC | IL | ||
| Number of plants PCR tested | 919 | 301 | 205 | 41 | 1,466 |
| Number of PCR positives | 84 | 9 | 15 | 8 | 116 |
| Number of plants screened by Southern blot assay | 53 | 6 | 5 | 1 | 65 |
DC De-embryonated cotyledon, IL Immature leaves
