Fig. 5.

Aqueous extract inhibits hemagglutinating activity of influenza virus. Cell culture with virus and different concentrations of the extract from different experiments pre exposure, simultaneous exposure and post exposure were added in 96-well plate with equal amount of guinea pig erythrocytes (0.75 %) and incubated for 1 h. We observed that at a concentration of 25 mg/mL; aqueous extract reduces HA activity of virus from 64 to no HA titre. When the concentration was further reduced to 0.025 mg/mL in simultaneous and post exposure analysis; HA activity reduced to 16 as compared to pre exposure where it was 64 HA titre