Figure 4. Active component is released after hatching and is necessary past 24 hours to affect survival.

N2 L1 worms were starved continuously at high density in the same medium (control HD), at high density but with a wash and fresh buffer after the first 24 hours of starvation (washed HD), continuously at low density (control LD), at high density for the first 24 hours and then diluted to low density (HD to LD).