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. Author manuscript; available in PMC: 2014 Aug 6.
Published in final edited form as: Anal Chem. 2013 Jul 23;85(15):6995–7002. doi: 10.1021/ac4012655

Figure 1.

Figure 1

Schematic describing the basic steps involved in the CIU assay to determine kinase inhibitor binding modes. Protein ligand complexes are ionized by nESI in a range of charge states (A), a single charge state is selected for activation in a quadrupole mass filter (B). Following selection, collisional activation with argon (varying the amount of accelerating voltage) is used to initiate three different processes: charge stripping, inhibitor dissociation, and gas-phase protein unfolding (C). IM drift time is monitored and recorded over a broad range of collision voltages to create a complete CIU fingerprint, which creates a contour plot of the intensity of ion populations as a function of these two parameters (D).