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. 2000 Jun 15;105(12):1731–1740. doi: 10.1172/JCI8472

Figure 4.

Figure 4

Inhibition of EO6 binding to Cu-OxLDL by PC, as sodium salt (PC-Cl) or PC-KLH conjugate. Cu-OxLDL (10 μg/mL) was plated on microtiter wells overnight at 4°C. EO6 (10 μg/mL) was added to wells in the absence or presence of the indicated concentrations of competitors, and the amount of bound EO6 was detected by AP-conjugated goat anti-mouse IgM. The amount of bound EO6 was expressed as the percent of EO6 binding to Cu-OxLDL in the absence of competitor. Inset: Inhibition of EO6 binding to Cu-OxLDL by both EO6 and T15. Cu-OxLDL (10 μg/mL) was coated on microtiter wells overnight at 4°C. Biotinylated EO6 (10 μg/mL) was added to microtiter wells in the absence or presence of the indicated concentrations of competitors. The amount of biotinylated EO6 bound to Cu-OxLDL was then detected by AP-conjugated NeutrAvidin®. The amount of biotinylated EO6 bound to the antigen in the absence of competitor was expressed as a percentage of the control. Nonspecific mouse IgA was used as isotype control for T15.