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. Author manuscript; available in PMC: 2013 Sep 27.
Published in final edited form as: Immunity. 2013 Jan 3;38(2):263–274. doi: 10.1016/j.immuni.2012.09.011

Figure 2. Polyclonal CD4+ T cells show a broad range of self-reactivity that is correlated with CD5 expression.

Figure 2

(A, D, and F) Immunoblot analysis for tyrosine phosphorylation of the TCR ζ chain and total ERK2 in naïve polyclonal CD5hi and CD5lo CD4+ T cells, (A); polyclonal CD8+ T cells, (D); and 5C.C7 TCR Tg CD4+ T cells, (F). RDV relative to the CD5lo population is given below each lane. Sort stringencies are for the 10% highest or lowest populations unless specified otherwise. Representative data from 2–3 experiments are shown. (B) Correlation between CD5 RFI and phospho-ζ RDV for CD4+ T cells sorted on CD5 expression. RDVs are normalized relative to unsorted CD4+ polyclonal cells. Data are from 5 independent experiments. (C and D) CD5 coefficient of variation for polyclonal CD4+ and CD8+ T cells, (C); polyclonal or TCR Tg CD4+ T cells (E). ns, not significant.