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. Author manuscript; available in PMC: 2014 Aug 5.
Published in final edited form as: Mol Pharm. 2013 Jul 8;10(8):3175–3185. doi: 10.1021/mp400222j

Figure 2.

Figure 2

In vivo uptake studies of the MC1RL-Cy5 probe using the dorsal skin-fold window chamber xenograft tumor model for intravital confocal imaging. A) Mouse bearing a dorsal skin-fold window-chamber. B) Verification of GFP rat microvessel (green) patency following intravenous injection of Blue Dextran using confocal fluorescence microscopy. C) Intravital confocal microscope image of probe (red) labeling tumor cells 24 h after administration and green microvessels using 4X magnification. Bar = 1000μm. D) At 25X magnification (left panel for each time-point), probe fluorescence was detected on the cell surface by 5 min after i.v. injection of the probe, and was detected inside the cells by 24 h after injection. A close-up image (right panel for each time-point) is shown to better illustrate cell binding and uptake. Bar = 100μm. E) Quantification of the probe-related fluorescence signal before injection and at 24 hr after injection (P=0.025). Representative images are underneath each graph.