. Author manuscript; available in PMC: 2014 Aug 5.

Published in final edited form as: Mol Pharm. 2013 Jul 8;10(8):3175–3185. doi: 10.1021/mp400222j

Table 2.

Parameter value estimates by the multi-compartmental pharmacokinetic computational model derived from ex vivo experiments.

Parameter^*	Mean±s.d. of 10 simulations
K_ti (NU^**.h)⁻¹	2.7+1.4
K_to (h)⁻¹	26+12
K_ki (NU.h)⁻¹	1E-2+6.8E-3
K_b (h)⁻¹	7.8E-2+1.4E-2
VB (cm³)	1.4 ^†
VT (cm³)	0.8 cm³^†
VK (cm³)	0.9 cm³^†
VN (cm³)	25 cm³^†
K_ni (NU.h)⁻¹	1.8+0.2
K_no (h)⁻¹	2.3+0.6
T_MAX (NU)	130+9
K_MAX (NU)	2.7E3+2.3E3
K_ko (h)⁻¹	0.96+0.14

Each parameter is defined in the materials and methods section.

^†

Denotes parameters whose values were obtained from weighing the mouse organs ex vivo, and were fixed in the beginnig of the simulation, thus no standard deviation exists. VB is the volume of blood, VT is the volume of the MC1R high expressing tumor, VK is the combined volume of both kidneys, and VN is the total volume of the mouse minus kidneys, blood and MC1R+ tumor.

^**

NU stands for Normalized Unit of fluorescence measured, which is proportional to ligand concentration.