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. Author manuscript; available in PMC: 2013 Sep 27.
Published in final edited form as: J Histochem Cytochem. 2003 May;51(5):675–685. doi: 10.1177/002215540305100513

Figure 5.

Figure 5

S100A8 and S100A9 do not form crosslinked multimers. (A) Equivalent quantities of protein (20 μg/lane), prepared from normal epidermis (patient N1) or from uninvolved and involved psoriatic epidermis (patient P3), were electrophoresed on a denaturing 12% polyacrylamide gel, transferred to nitrocellulose, and incubated with anti-S100A8 (1 μg/incubation) in the absence or presence of S100A8 epitope-specific blocking peptide (5 μg/incubation). (B) An identical parallel blot was incubated with anti-S100A9 (1 μg/incubation) in the presence or absence of S100A9 epitope-specific blocking peptide (5 μg/incubation). β-Actin levels were monitored to normalize gel loading. Arrows indicate the migration of the S100A8 (A) or S100A9 (B) monomer.