Table 1.
Frequency of HLA-identical sibling mHA-specific CD4 T cells in peripheral blood
| HLA-Id sibling pair | Treg | Tconv | mHA-specific Tconv/Treg ratio | ||||
|---|---|---|---|---|---|---|---|
| % of total CD4* | Frequency† | 95% CI | % of total CD4* | Frequency† | 95% CI | ||
| 1 | 7.5 | 0.5 | 0.2-0.7 | 92.5 | 20.4 | 12.0-34.2 | 39 |
| 2 | 3.6 | 0.4 | 0.1-1.4 | 96.4 | 8.7 | 2.9-22.2 | 24 |
| 3 | 7.9 | 0.9 | 0.4-2.0 | 92.1 | 23.0 | 13.8-35.9 | 26 |
| 4 | 6.8 | 0.8 | 0.3-1.9 | 93.2 | 25.2 | 14.9-42.9 | 31 |
| 5 | 7.7 | 0.9 | 0.3-2.9 | 92.3 | 24.9 | 15.7-40.6 | 27 |
| 6 | 14.3 | 2.0 | 0.9-4.6 | 85.7 | 23.1 | 13.7-37.7 | 12 |
| 7 | 4.9 | 1.1 | 0.4-2.5 | 95.1 | 34.2 | 15.2-77.0 | 32 |
| 8 | 3.5 | 0.9 | 0.6-1.6 | 96.5 | 25.1 | 14.5-42.5 | 27 |
| 9 | 5.2 | 1.6 | 0.7-3.7 | 94.8 | 41.7 | 19.9-87.2 | 27 |
| 10 | 9.4 | 3.0 | 1.6-5.6 | 90.6 | 25.4 | 13.6-48.9 | 8 |
| 11 | 6.6 | 2.8 | 2.5-4.3 | 93.4 | 42.0 | 25.2-71.0 | 15 |
| 12 | 6.4 | 3.3 | 1.9-5.4 | 93.6 | ND | — | — |
| 13 | 7.7 | 5.4 | 3.2-9.0 | 92.3 | 68.3 | 41.5-113.5 | 13 |
| 14 | 9.2 | 8.4 | 5.2-13.5 | 90.8 | 89.0 | 55.4-141.6 | 11 |
| 15 | 8.3 | 1.6 | 0.7-3.7 | 91.7 | 29.3 | 14.7-58.7 | 19 |
| 16 | 14.7 | 6.7 | 3.7-12.8 | 85.3 | 63.1 | 37.5-104.1 | 9.3 |
| Median | 7.6 | 1.6 | 92.4 | 25.4 | 24 | ||
| Range | 3.5-14.7 | 0.4-8.4 | 85.3-96.5 | 8.7-89 | 8-39 | ||
HLA-Id, HLA-identical; ND, not determined.
*
Percentage Treg (CD4+CD25+CD127−) or Tconv (CD4+CD25−CD127+) was calculated based on flow cytometry analysis of unseparated blood samples.
†
Treg ands Tconvs were enriched to >95% purity before the assay. Values shown are the frequency of mHA-specific Tregs or Tconvs per million total CD4 T cells with 95% confidence intervals. mHA-specific Treg or Tconv frequencies were obtained from LDA of purified population in culture with DCs from HLA-identical siblings in the presence of IL-2, IL-15, and rapamycin (Tregs) or IL-2 alone (Tconvs).