Figure 3. Retinal ganglion cell growth cone guidance by En-2 is dependent on protein synthesis.

a, Temporal (red) and nasal (black) growth cones isolated from their somas (see Supplementary Fig. 1c) show opposite turning responses to an En-2 gradient, eliminating a role for transcription. b, ³H-leucine incorporation in growth cones isolated from their cell bodies. En-2 stimulates significant incorporation of ³H-leucine, which is abolished by anisomycin but not α-amanitin. c, Mean turning angles of temporal and nasal growth cones in a gradient of En-2 in the presence of inhibitors. En-2-induced turning is blocked by anisomycin and rapamycin, but is unaffected by α-amanitin. The number of growth cones tested in each case is indicated on the bars. b, Significance was calculated using a Kruskal–Wallis test, and indicated by asterisks (*P < 0.05; **P < 0.01). c, Significance was calculated using a Kolmogorov–Smirnov test, and indicated by asterisks (*P < 0.05; **P < 0.01; ***P < 0.001), and comparisons are to En-2. Error bars indicate s.e.m.