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A representative 2-D gel image of hypobaric hypoxia exposed rat plasma following albumin and IgG depletion. Proteins (500 μg) were separated by IEF using pH 4–7, 18 cm IPG strips (GE Healthcare, Sweden) and 12% SDS PAGE. The gels were silver stained and analyzed by Progenesis Same spots 2D gel image analysis software (Nonlinear Dynamics). Spot numbers represent differentially expressed proteins (±1.5-fold, p<0.05) among tolerant and susceptible compared with control.
