Expression of human thrombomodulin (TBM) and CD39 by GalT KO/CD55/CD59/HTF pig fibroblasts stably transfected with 3-gene construct #4 (H-2Kb/Hygro-TBM-CD39). (A) pooled transfectants stained for TBM, showing the gates used to sort populations for expansion. (B) pooled transfectants stained for CD39. Black line, untransfected cells (C) and (D), expanded sorted populations stained for expression of TBM and CD39, respectively. High, medium, low and negative TBM-expressing pools are indicated by green, blue, purple and red lines.