Table 1.
Oligonucleotides and primers used in the construction of multi-gene cassettes
| Name | Sequence |
|---|---|
| fuF2A-1 | CTAGAGCCAAACGCGCTCCCGTGAAGCAGACCCTGAACTTTGACCTTCTGAAACTTGCCGGCGACGTCGAGTCCAACCCTGGCC |
| fuF2A-2 | CCGGGGCCAGGGTTGGACTCGACGTCGCCGGCAAGTTTCAGAAGGTCAAAGTTCAGGGTCTGCTTCACGGGAGCGCGTTTGGCT |
| 55Xba-F | CCCTTCTAGAATTCGCTGCGACTCGGCGGAGT |
| 55Xba-R | CCCTTCTAGAAGTCAGCAAGCCCATGGTTACTA |
| 39Xma-F | CCCCCGGGATGGAAGATACAAAGGAGT |
| 39Xho-R | CCCCCTCGAGAATTCATACCATATCTTTCCAGAAAT |
| TBM-F | ATGCTTGGGGTCCTGGTCCTTGGCGCGCTGGCCCTGGCCGGCCTGGGGTTCCCCGCACCCGCAGAGCCGCAGCCGGGTGGCAGCCAGTGCGTCGAGCACGACTGCTTCGCGCTCTATCCGGGCCCCGCG |
| TBM-R | GGGTCCGGGATTCTCCTCCACGTCGCCGCAGGTCAGCAGGCTGCCGCGGCCCTCGCGCTTGGCTCTGAGTCTCTGCGGCGTCCGCTCGGT |
| LEA-F1 | ATGGCTTGCCTTGGATTTCAGCGGCA |
| LEA-F2 | TCTCCAGGCAAATACACTGAGGTCCGGGT |
| LEA-F3 | ACCGCCATACTACGAGGGCATAGGCA |
| LEA-F4 | TTTGGTACCATGGCTTGCCTTGGATTTCAGCGGCA |
| LEA-R1 | ACCCGGACCTCAGTGTATTTGCCTGGAGA |
| LEA-R2 | TGCCTATGCCCTCGTAGTATGGCGGT |
| LEA-R3 | AGGTCCGGGGTTGCTTTCCACATCGCCAGCGAGTTTCAACAAAGCGTAGTTAGTACACTGCCGCTTAGCCCTTTTACCCGGAGACAGGGAGAGGCT |
| LEA-R4 | AGGTCCGGGGTTGCTTTCCACATCGCCA |
| LEA-R5 | GGGATCCGGGTCAGAATCTGGGCACGGTTCTGGATCAATTACATAAATCTGGGTTCCGTTGCCTATGCCCTCGTAGT |
TBM, thrombomodulin.