Skip to main content
. 2013 Aug 27;2(10):758–765. doi: 10.5966/sctm.2012-0156

Figure 2.

Figure 2.

The effects of EGF, KGF, and Y-27632 on the morphology of human limbal epithelial cell sheets. (A): Phase contrast micrograph of cells cultured for 1 month in the indicated medium. (B–D): Cryosections stained with antibodies specific for K15 (green, [B]), K12 (red, [B]), K3 (green, [C]), PAX6 (red, [C]), p63 (green, [D]), and CDH1 (red, [D]). Phase contrast images were merged with immunofluorescence images. Where indicated, cell nuclei were stained with DAPI. Culture conditions were the same as for the top panel. Scale bars = 100 μm (A) and 50 μm (B–D). Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; E+Y, epidermal growth factor and Y-27632; EGF, epidermal growth factor; K+Y, keratinocyte growth factor and Y-27632; KGF, keratinocyte growth factor; mo, month.