Figure 2.

Total and apically expressed Ncc protein was dramatically attenuated in the distal convoluted tubules (DCTs) of Ncc T58M knock-in mice. Representative immunogold labeling of Ncc in DCT cells of (A) WT and (B) homozygous Ncc T58M knock-in mice (Ho), respectively. Arrows (→) and arrowheads (►) indicate the apical and cytosol located Ncc (x22.5k), respectively, in DCTs. (C) Semi-quantification of immunogold labeling of Ncc. Total and apical Ncc signals in WT and Ho mice are shown in the figures (n=6/group).