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. 2013 Jul 5;24(10):1587–1597. doi: 10.1681/ASN.2012070742

Figure 4.

Figure 4.

Blunted Ncc but augmented Nkcc2 and ENaC function in the Ncc T58M knock-in mice. Effect of hydrochlorothiazide (HCTZ), furosemide (FURO), and amiloride (AMI) administration on urine amount and Na+, K+, and Cl− excretion. HCTZ (12.5 mg/kg), FURO (15 mg/kg), or AMI (5mg/kg) is intraperitoneally administered to WT and NccT58M/T58M (Ho, ▪) littermates (n=6/group) and urine samples for the 4 hours after a single dose is collected for analysis. FENa, FEK, and FECl represent the fractional excretion of Na+, K+, and Cl−, respectively. (A) In response to HCTZ, significantly increased urine amount, FENa, FEK, and FECl are observed in WT but blunted in Ho mice. (B) In the case of FURO (15 mg/kg), exaggerated responses of urine amount, FENa, FEK, and FECl are observed in Ho mice. (C) In the case of AMI, exaggerated responses of urine amount, FENa, and FECl but not FEK are observed in Ho mice.