Figure 7.

Ncc T58M mutation resistant to activated Spak stimulation in vivo. Semiquantitative immunoblotting of p-Spak, total Ncc, and p-Ncc expression. (A) Low Na⁺ (L) diet enhances Spak phosphorylation in both WT and Ncc T58M homozygous knock-in mice. However, enhanced total and p-Ncc abundance are only observed in WT rather than Ncc T58M homozygous knock-in mice. *P<0.05 L diet versus normal Na⁺ (N) diet. (B) Constitutive activation of Wnk4-Spak signaling cannot rescue the expression of Ncc in Ncc T58M homozygous with Wnk4 D561A heterozygous double knock-in mice (n=3/group). *P<0.05 versus WT.