Figure 7.
IL-11 is critical for A1AR-mediated induction of SK-1 in HK-2 cells. SK-1 and SK2 mRNA (RT-PCR) (A) and protein (immunoblotting) (B) expression in HK-2 cells treated with vehicle or with 1 μM CCPA for 6 hours (n=4–5). Representative images (top) and band intensity quantifications (bottom) expressed as fold increases in SK-1 or SK2 expression over vehicle-treated cells. HK-2 cells were pretreated with control isotype antibody (IgG) or with IL-11–neutralizing antibody (10 μg/ml) 30 minutes before vehicle or CCPA treatment. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA and β-actin protein expression were also quantified to normalize lane loading. *P<0.05 versus vehicle-treatment cells pretreated with IgG isotype antibody. #P<0.05 versus CCPA-treated cells pretreated with IgG isotype antibody. Error bars represent 1 SEM.