Table 1.
Primers used to amplify cDNAs based on published GenBank sequences for mice.
| Primers | Accession Number | Sequence (Sense/Antisense) | Product Size (bp) | Cycle Number | Annealing Temp (°C) |
|---|---|---|---|---|---|
| Mouse IL-11 | NM_008350.4 | 5′-AACTGTGTTTGTCGCCTGGT-3′ | 267 | 30 | 68 |
| 5′-AAGCTGCAAAGATCCCAATG-3′ | |||||
| Mouse SK1 | NM_011451 | 5′-GATGCATGAGGTGGTGAATG-3′ | 337 | 22 | 64 |
| 5′-GCCCACTGTGAAACGAATC-3′ | |||||
| Mouse SK2 | NM_020011.5 | 5′-ACTGCTCGCTTCTTCTCTGC-3′ | 437 | 23 | 68 |
| 5′-ACCATTGAGGGACAGGTCAG-3′ | |||||
| Human IL-11 | NM_000641.3 | 5′-CTGAAGACTCGGCTGTGACC-3′ | 300 | 22 | 66 |
| 5′-CAGGGCAGAAGTCTGTGGAC-3′ | |||||
| Human SK1 | NM_021972 | 5′-ATCTCCTTCACGCTGATGC-3′ | 330 | 26 | 66 |
| 5′-GTGCAGAGACAGCAGGTTCA-3′ | |||||
| Human SK2 | NM_020126 | 5′-GGAGGAAGCTGTGAAGATGC-3′ | 482 | 22 | 66 |
| 5′-GCAGGTCAGACACAGAACGA-3′ | |||||
| GAPDH | M32599 | 5′-ACCACAGTCCATGCCATCAC-3′ | 450 | 15 | 65 |
| 5′-CACCACCCTGTTGCTGTAGCC-3′ | |||||
| A1ARloxP | 5′-GCCTTCCGAATCCACAGTT-3′ | Lox: 450 | 35 | 62 | |
| 5′-AGCCTCTGTTCCACATACAC-3′ | WT: 1350 | ||||
| Cre transgene | 5′-TGGGCGGCATGGTGCAAGTT-3′ | 466 | 30 | 61 | |
| 5′-CGGTGCTAACCAGCGTTTTC-3′ |
GAPDH, glyceraldehyde 3-phosphate dehydrogenase.