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. Author manuscript; available in PMC: 2014 Apr 25.
Published in final edited form as: Cell Rep. 2013 Apr 18;3(4):1164–1174. doi: 10.1016/j.celrep.2013.03.028

Figure 4. Fat Progenitor Cells of BubR1 Hypomorphic Mice Are Senescence Prone.

Figure 4

(A) SA-β-gal-positive cells from IAT accumulate in regions that track along blood vessels (red dotted line) and not in mature adipocytes of 5-month-old BubR1H/H mice. Scale bars represent 1 mm (left) and 100 μm (right).

(B) Quantitation of ASCs/PACs in IAT of 2-month-old mice. Values are expressed as percent of Sca1+ cell numbers to account for differences in input material among genotypes.

(C) qRT-PCR analysis for p16Ink4a expression in sorted adipose cells from 2-month-old wild-type and BubR1H/H mice. Values are normalized to wild-type endothelial cells (Endo). AC, adipocyte.

(D) qRT-PCR analysis for senescence marker expression in ASCs/PACs from 2-month-old mice relative to wild-type cells.

Data in (B)–(D) are mean ±SD (n = 3 mice per genotype). Asterisks denote significant changes from BubR1H/H values. *p < 0.05; **p < 0.01. See also Figure S3.