Figure 6. p53 Delays while p21 Drives Cataractogenesis in BubR1 Progeroid Mice.

(A) Incidence and latency of cataract formation in BubR1^H/H, BubR1^H/H;p53^−/−, and BubR1^H/H; p21^−/− animals as detected by use of slit light after dilating the eyes.

(B) Hematoxylin and eosin-stained lenses of 2-month-old mice. Arrowheads indicate posteriorly located lens epithelial cells. Asterisks mark Morgagnian globules. Scale bar represents 100 μm.

(C) Quantitation of the average number of posteriorly located cells (defined as cells that have migrated past the lens epithelial bow) in lenses of the indicated genotypes. Data are mean ±SD (n = 4 mice per genotype, two lenses per mouse). We note that no cells are detectable in this area of wild-type mice.

(D) qRT-PCR analysis of p16^Ink4a and p19^Arf expression in eye tissue of 2-month-old mice. Data are mean ±SD (n = 5 mice of each genotype). (E) qRT-PCR analysis on lens tissue of 6-month-old wild-type and BubR1^H/H mice. Data are mean ±SD (n = 10 mice per genotype).

Asterisks denote significant differences compared to BubR1^H/H mice. *p < 0.05; **p < 0.01; ***p < 0.001. See also Figure S4.