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. Author manuscript; available in PMC: 2013 Sep 27.
Published in final edited form as: Cell Rep. 2012 Aug 2;2(2):294–307. doi: 10.1016/j.celrep.2012.06.018

Figure 4. ovo is required for formation of regenerative eye progenitors, and ovo+ progenitor presence requires other eye transcription factors.

Figure 4

(A)–(B) ovo RNAi eliminates regenerative eye progenitors as assessed by markers for PRN (otxA+/eya+), PC (sp6-9+/eya+), and general eye progenitors (six-1/2+/eya+). Arrows indicate double-positive cells.

(C)–(D) Most regenerative ovo+ progenitor cells are eliminated by six-1/2 and eya RNAi.

(E)–(F) sp6-9 RNAi eliminates most ovo+ pigment cell progenitors (ovo+/otxA), and otxA RNAi eliminates most ovo+ photoreceptor neuron progenitors (ovo+/sp6-9).

n = 8 eyes + trails for all quantifications.

Error bars represent SD. *** p < .005. Scale bars, 50 µm (A,C,E), and 20 µm (A, insets).