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. Author manuscript; available in PMC: 2013 Sep 27.
Published in final edited form as: Cell Rep. 2012 Aug 2;2(2):294–307. doi: 10.1016/j.celrep.2012.06.018

Figure 5. ovo is expressed in a population of homeostatic eye progenitors.

Figure 5

(A) In starved intact animals, ovo is expressed in isolated dorsal cells between the eyes and pharynx. These cells are eliminated within 24 hours of irradiation.

(B) ovo+ cells posterior to the eyes in intact animals also express six-1/2 and eya.

(C) ovo+ cells posterior to the eyes in intact animals also express the neoblast marker histone h2b (60% +/− 15%, α =0.05).

(D) ovo(RNAi) animals that are not amputated fail to maintain eyes (n=10/10).

(E)–(F) ovo(RNAi) animals that are not amputated fail to maintain six-1/2+/eya+ cells posterior to the eyes (n=4 animals). For image clarity only six-1/2 signal is shown.

(G)–(H) six-1/2(RNAi) and eya(RNAi) animals fail to maintain ovo+ cells posterior to the eyes (n=4 animals).

Arrows indicated double-positive cells in (B) and (C). Error bars represent SD. *** p < .005. Scale bars, 100 µm (A), 50 µm (B,C,F,H), and 200 µm (D).