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. Author manuscript; available in PMC: 2013 Sep 27.
Published in final edited form as: Cell Rep. 2012 Aug 2;2(2):294–307. doi: 10.1016/j.celrep.2012.06.018

Figure 6. Smed-soxB, Smed-meis, Smed-klf, and Smed-foxq2 are required for eye regeneration.

Figure 6

(A) Eye regeneration defects at 7 days of regeneration in live RNAi animals.

(B) Detection of tyrosinase transcript and Arrestin protein reveals defects in optic cup formation and photoreceptor neuron regeneration. Arrow indicates duplicated optic cup in meis(RNAi) animal.

(C) Quantification of cross-section area (µm2) of photoreceptor neuron aggregate (mean + SD, n=8 eyes).

(D) soxB RNAi results in loss of anterior subtypes of photoreceptor neurons, but not posterior subtypes (n=8 eyes for each condition).

(E)–(F) soxB RNAi does not affect numbers of ovo+ progenitors, nor the ratio of PC progenitors (sp6-9+/ovo+) to PRN progenitors (sp6-9/ovo+).

Error bars represent SD. *** p < .005. Scale bars, 200 µm (A), 100 µm (D), 20 µm (B, E).