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. Author manuscript; available in PMC: 2013 Sep 27.
Published in final edited form as: Clin Cancer Res. 2011 Nov 7;18(1):184–195. doi: 10.1158/1078-0432.CCR-11-1558

Figure 2.

Figure 2

Molecular responses of human glioma cells to NVP-BKM120. A and B, cell lysates were separated by gel electrophoresis and immunoblotted for phospho-Akt (Ser473), phospho-Akt (Thr308), phospho-S6 (Ser240/244 and Ser235/236), and phospho-4E-BP1 (Thr37/46). Total Akt, total S6, total 4E-BP1, and actin were used as loading controls. C, representative cell-cycle profiles of glioma cells following 72 hours exposure to IC50 concentration of NVP-BKM120 represented as stacked columns (*, P < 0.04; **, P < .0001; #, P < 0.0001; ##, P < 0.0001). D, accumulation of multinucleated cells in U251 cells exposed to increasing concentrations of NVP-BKM120. Scale bars, 25 μm. E, quantification of multinucleated cells after NVP-BKM120 in glioma cells. Con, control.