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. 2013 Sep 27;8(9):e76016. doi: 10.1371/journal.pone.0076016

Figure 6. p62 regulates physiological function of cortactin-F-actin assembly.

Figure 6

(A) WT MEF cells, either untreated (a-f) or treated (g-l),with 5 µM MG132 for 6 hours were fixed with paraformaldehyde. F-actin was stained with phalloidin-AF350. Endogenous cortactin was immuno-stained with Texas Red secondary antibody and endogenous HDAC6 immuno-stained with Oregon Green secondary antibody. Cells were analyzed with confocal microscopy. Panel identification is as follows: a and g – actin; b and h – cortactin; c and i – HDAC6; d and j – actin/cortactin; e and k – actin/HDAC6; f and l – cortactin/HDAC6. A fully merged image is shown to the right of each set of panels. (B) p62KO MEF cells, either untreated (a-f) or treated (g-l), with 5 µM MG132 for 6 hours, were subjected to the same immunofluorescence procedure as described above.