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Yeast complementation experiment with BCS1L. The BCS1 deletion yeast strain was transformed with the wild-type and mutant BCS1L constructs in a high-copy yeast expression plasmid pRS425. The wild-type yeast BCS1 cDNA in the same vector was used as a positive control, and the plain vector was used as a negative control. A, YPEG (ethanol-glycerol) plate incubated at 30°C for 11 d. B, YPEG plate incubated at 37°C for 8 d. C, YPD (glucose) plate incubated at 30°C for 3 d.
