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. 2013 Sep 27;8(9):e76191. doi: 10.1371/journal.pone.0076191

Figure 5. Apoptosis: Number of Apoptotic cells (cells with nuclear fragmentation and/or with distinct apoptotic bodies) per hundred cells.

Figure 5

(A) Control (B) Vehicle control (C) Cu (II) (25µM) [added to non calcitriol loaded, control lymphocytes] (D) Calcitriol overload (E) Calcitriol overload + Cu (II) (25µM) (F) Calcitriol overload + Cu (II) (25µM) + Bathocuprione (50µM) (membrane impermeable Cu(II) chelator) (G) Calcitriol overload + Cu (II) (25µM) + Neucuprione (50µM) (membrane permeable Cu(II) chelator) (H) Calcitriol overload + Cu (II) (25µM) + Desferoxamine (50µM) (Fe(II) chelator) (I) Calcitriol overload + Cu (II) (25µM) + Histidine (50µM) (Zn(II) chelator) (J) Calcitriol overload + Cu (II) (25µM) + SOD (20µg/ml) (K) Calcitriol overload + Cu (II) (25µM) + Catalase (20µg/ml) (L) Calcitriol overload + Cu (II) (25µM) + Thiourea (0.1mM). Values expressed as mean + SEM (n=3). P < 0.05 when compared to control and COLs with Cu (II) (25µM). All incubations were carried out for 2 hours at 37º C.