RAW 264.7 cells were plated and treated with OmpU or protein-buffer and incubated for 24 h. After 24 h of OmpU or protein-buffer treatment, cells were re-plated in fresh media without PmB and challenged with LPS and incubated for 8 h. Cells were harvested and total RNA was isolated. Semi quantitative RT-PCR was performed to evaluate changes in mRNA expression of Nos2, Tnfα and Il6. Results are expressed as mean ± SEM and represent the average of three independent experiments. *p< 0.05, **p<0.01 ***p < 0.001 versus LPS.
A. Suppression of LPS mediated Nos2 expression was observed in RAW 264.7 cells pre-treated with OmpU.
B. Tnfα expression was suppressed in LPS activated RAW 264.7 cells pre-treated with OmpU.
C. OmpU pre-treatment suppressed LPS mediated of Il6 expression in RAW 264.7 cell line.