Figure 4. Cynomolgus macaques (n=3 per dose group) were dosed with a single dose of 0.01 mg/kg, 0.03 mg/kg, 0.1 mg/kg and 0.3 mg/kg of NKTT120, respectively and subsequently bled at indicated time points.

Whole blood samples were stained with antibodies against CD3 (SP34.2), CD20 (2H7), TCR Vα24 (C15), CD159a (Z199) and invariant TCR (6B11). Subsequently red blood cells lysed, cells washed and analyzed by flow cytometry. T cells were identified as CD20-, CD3+ and 6B11- lymphocytes, B cells were identified as CD3-, CD20+ lymphocytes and NK cells were identified as CD3-CD159a+ lymphocytes. T, B and NK cells are reported as % of lymphocytes One way ANOVA with multiple comparison followed by a non-parametric T test was performed using PRISM software on all the subsets. * indicates significant difference to pre-bleed values (P=0.01).