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. 2013 Sep 27;8(9):e76084. doi: 10.1371/journal.pone.0076084

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© 2013 Cejudo-Román et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Flow cytometry plots of spermatozoa after overnight labeling with a Na _v1.8 antibody (red plot) and the negative control treated with secondary antibody alone (black plot), n = 9. (B) Immunofluorescence images of sperm cells stained with a primary antibody against Na _v1.8. Bar graph represents the distribution of this voltage-gated sodium channel in different sperm regions and the percentage of sperm cells showing each specific localization, n = 9. Scale bar, 10 μM. (C) Western blot analysis of Nav1.8 in human sperm homogenates using the Nav1.8 rabbit polyclonal antibody ab-66743. Molecular mass is indicated on the right side of the panel. The figure is representative of results obtained in 6 separate protein preparations from 6 different donors.