Skip to main content
. 2013 Sep 27;8(9):e76084. doi: 10.1371/journal.pone.0076084

Figure 5. Time-dependent effects of veratridine on sperm hyperactivation and acrosome reaction (AR).

Figure 5

(A) Effect of veratridine (10 μM) and its solvent on hyperactivated sperm motility. Capacitated sperm were treated with veratridine or its solvent for different times and hyperactivation evaluated by computer-assisted sperm analysis (CASA). Bars are means with SEM of 7 different experiments and represent % hyperactivated sperm. (B) Effect of veratridine and A-23187 on AR. Capacitated sperm were treated with veratridine (10 μM) or A-23187 (10 μM) for different times and the acrosomal status was assessed by staining sperm with FITC-PSA. Bars are means with SEM of 5 different experiments and were calculated as: (%AR reacted spermatozoa in veratridine- or A23187-treated aliquots) – (%AR reacted spermatozoa in the corresponding solvent-treated aliquots at the same time). * P<0.05, significant difference vs. values at time =0.