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. 2013 Sep 27;8(9):e76078. doi: 10.1371/journal.pone.0076078

Figure 5. Lymphatic flow measurement using near infrared dye ICG.

Figure 5

A-D Mice were injected I.D. with IGC at the base of the tail to quantify the contractility and qualify the ejection fraction of the lymph at days 0(A), 3(B), and 7(C) of post-CHS in the contralateral view at day 3(D) (N=3). Selected frames are showed from the same sample mice to illustrate the changes that occur in the lymphatic flow. Spatio-temporal fluorescent intensity maps are shown to visualize the ejection fraction of the ICG dye. We observed that mice pre-treatment (A), at day 7 post-CHS (B) or the contralateral side at day 3post-CHS (D) have forward flow, but that at day 3 (C) on the side of inflammation the lymph flow is perturbed and shows backflow.