Figure 1. Co-immunoprecipitation of S100A14 and S100A16 in oral cancer cells.

Five hundred micrograms of pre-cleared cell extract from CaLH3 cells was incubated with anti-S100A14 antibody in the presence of 0-2 mM CaCl₂ or 2 mM CaCl₂ with 5 mM EDTA (A) or anti-S100A16 antibody (B), followed by incubation with Protein A/G PLUS-Agarose. Immune complexes were immunoblotted with anti-S100A16 (A) or anti-S100A14 antibody (B). Only lysate (without any antibody) and anti-MMP9 antibody were used as controls for the Co-IP. (A) M, molecular weight marker; lane 1, input; lane 2, only lysate; lane 3, control anti-MMP9 antibody; lanes 4-7 (anti-S100A14 antibody): lane 4, 0 mM CaCl₂; lane 5, 0.5 mM CaCl₂; lane 6, 2 mM CaCl₂; lane 7, 2 mM CaCl₂ with 5 mM EDTA. (B) lane 1, input; lane 2, only lysate; lane 3, control anti-MMP9 antibody; lane 4, anti-S100A16 antibody with 0 mM CaCl₂.