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. 2013 Sep 27;8(9):e76058. doi: 10.1371/journal.pone.0076058

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© 2013 Sapkota et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Cellular localization of S100A14 and S100A16 was examined by performing DIF and IHC in the sections of formalin fixed and paraffin embedded OSCC and normal oral mucosal tissues. Predominantly membranous localization of S100A16 (green), S100A14 (red) and S100A16/A14 (yellow) was visualized in the epithelial cells of normal mucosa (A1-A4) and OSCC (B1-B4) tissues by DIF (Epth, epithelium; CT, connective tissue). Similarly, with single and double IHC on the serial sections, predominantly membranous expression of S100A16 (purple), S100A14 (gray) and S100A16/A14 (mixed color) was detected in the normal mucosa (C1-C3) and in the OSCC (D1-D3).