Skip to main content
. 2013 Sep 27;8(9):e76058. doi: 10.1371/journal.pone.0076058

Figure 3. Over-expression of S100A14 modulates expression of S100A16 protein but not mRNA in human cancer cell-lines.

Figure 3

(A) Retroviral mediated over-expression of S100A14 was associated with concomitant up-regulation of S100A16 protein in CaLH3, VB6, H357, OSCC1 and HeLa cell-lines (-, control construct; +, S100A14 expression construct). (B) Additionally, shRNA mediated knock-down of S100A14 in CaLH3 and OSCC1 cell-lines was associated with down-regulation of S100A16 protein (-, control construct; +, S100A14 shRNA constructs). (C) However, there was no effect in the mRNA expression levels of S100A16 by over-expressing S100A14 in CaLH3, VB6, and H357 cell-lines. S100A14 and S100A16 mRNA expression levels were normalized to GAPDH mRNA expression. Error bars represent SEM of 3 biological replicates done in 3 technical replicates. Student’s-t test (paired) was performed for statistical analysis.