Figure 2. Expression efficacy in immunohistochemically identified cell-types.

A) Confocal images of brain sections of the hippocampus prepared from mice that received injections of rAAV5 (left) and rAAV8 (right). Red channel: immunohistochemical label for GFAP, an astrocyte marker; blue channel: DAPI, labeling nuclei, green channel: GFP expression driven by viral vector. Examples of individual immunohistochemically identified cell bodies are marked with white arrowheads. B) Cumulative distributions of fluorescence measurements from individual cells expressed as ‘fold background’ (see methods). Individual lines correspond to a given analyzed section. Note, that cumulative distributions for astrocytes obtained from rAAV8 transduced mice are systematically shifted towards higher fluorescence levels as compared to rAAV5 transduced mice. C) Confocal images of brain sections of the auditory cortex prepared from mice that received injections of rAAV1 (left) and rAAV9 (right). Red channel: immunohistochemical label for NeuN, a neuron marker; blue channel: DAPI, labeling nuclei, green channel: GFP expression driven by viral vector. Examples of individual immunohistochemically identified cell bodies are marked with white arrowheads. D) Cumulative distributions of fluorescence measurements from individual cortical neurons expressed as fold background. All scale bars: 25 µm.