Figure 5. rAAV5 injections in the dentate gyrus lead to retrograde labeling of cells in the ventral entorhinal cortex.

A) Confocal images from horizontal brain sections at -3 mm along the dorso-ventral axis in reference to Bregma taken from a mouse that received a co-injection of rAAV5 driving expression of GFP (green) and fluorescently labeled retrograde tracer CTB (red) in the dentate gyrus. B) same as A), for Bregma -4.5 mm. C) same as A), for Bregma -6.0 mm. Double labeled cells in the ventral entorhinal cortex appear yellow. White squares on the left indicate parts of images shown in higher magnification on the right. Scale bars: for whole sections: 1 mm; for insets: 250 µm. D) Cell counts of CTB-only labeled cells (red), GFP-only labeled cells (green) and double positive cells (yellow) in horizontal sections of the ipsilateral entorhinal cortex along the dorso-ventral axis of three individual mice.