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. 2013 Sep 27;8(9):e77356. doi: 10.1371/journal.pone.0077356

Figure 3. Amounts of NGG and TUA1 transcripts in Pm35S:NGG, Pact1:NGG, and HSP:NGG lines.

Figure 3

(A, B) Transcript amounts of NGG (A) and TUA1 (B) in protonemata incubated for 8 days after propagation. Three independently transformed lines harboring a single copy of each DNA fragment at the PIG1 targeting site were analyzed (Figures S4 to S6). Copy numbers of the NGG and TUA1 transcripts per pg total RNA were estimated by RT-qPCR. In HSP:NGG lines, protonemata were cultured for 8 days at 25°C and collected after 1 h incubation at 25°C (crosses) or 38°C (squares).