Skip to main content
. 2013 Sep 27;8(9):e76234. doi: 10.1371/journal.pone.0076234

Figure 4. Gastrin treatment influences nucleus-cytosolic shuttling of NR4A2.

Figure 4

A: Intracellular localization of NR4A2 protein in response to gastrin treatment. AGS-GR cells transfected with pNR4A2-EGFP. B: Images of gastrin treated (10 nM) AGS-GR cells expressing pNR4A2-EGFP before, during and after bleaching of a nucleus area for 7.5 sec. The circle indicates the area of the bleach spot. C: Normalized FRAP curve for the cytosol of gastrin treated AGS-GR cells. D: Diffusion time in untreated and gastrin treated nucleus and cytosol. E: To determine cell viability, cells were transfected with pNR4A2-EGFP or a control plasmid (pH3.1-EGFP). After 48 h AGS-GR cells were detached by Accutase treatment, labeled with annexin V Alexa Fluor 647 and analyzed by flow cytometry. Annexin-V positive cells were considered as apoptotic. Results are shown as % apoptotic cells of the total number of counted EGFP positive or EGFP negative cells. Data are representative of three biological replicas; mean ± SD of three technical replicas is shown.