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. 2013 Sep 27;8(9):e76234. doi: 10.1371/journal.pone.0076234

Figure 5. NR4A2 suppresses gastrin-induced migration and invasion.

Figure 5

A: Real-time cell migration monitored (0-24 h) in AGS-GR cells transfected with siNR4A2 or siCtr, with or without gastrin treatment (10 nM). Results show one representative of three biological replicas; mean ±SD of three technical replicas. B: Invasion assay with AGS-GR cells transfected with pCMX-NR4A2 or pCMX (control) was performed in 24-well plates containing 8-µm pore Matrigel-coated inserts (with or without 0.3 nM gastrin). Cells invading the lower surface of the membrane were stained with Reastain Quick-Diff reagents and total numbers of cells in 5 fields per membrane were counted. The mean of three independent experiments is shown.